AFSC/RACE/EcoFOCI - Walleye Pollock larvae condition data measured in support of EcoFOCI assessment surveys and ecosystem observations in the southeastern Bering Sea and western Gulf of Alaska from 2007 to 2014

The datasets contain larval size, water temperature, cell cycle data, and classification of larval condition.

The datasets were developed to assess the condition of Walleye Pollock larvae using larval size, water temperature, and cell cycle data measured with flow cytometry.

Supplemental Information - Data Parameters and Units - Descriptive Information about the Data Parameters and Units.

The datasets contain larval standard length measured in mm, the proportion of cells in the S and G2/M phases of the cell cycle, and the ratio of the number S phase nuclei to the number of G1 phase nuclei with high nRNA content measured with flow cytometry. Also included is the mean water temperature (°C) between the surface and 40 m depth.

Supplemental Information – Methods - Descriptive Information about the methods used.

Larvae were collected using a 60 cm bongo frame fitted with 0.505 mm mesh nets and were frozen at -80°C. Tissue preparation, flow cytometry protocol, and data analysis are described in Porter and Bailey (2011 and 2013).

Supplemental Information – Instruments - Descriptive Information about the instruments and equipment used.

A BD Biosciences Influx flow cytometer (BD Biosciences, San Jose, CA) was used to analyze each larva. The proportion of nuclei in the S, and G2/M phases was calculated for each larva with MultiCycle AV software, vers. 4.0 (Phoenix Flow Systems, San Diego, CA). FCS Express flow cytometry analysis software, vers. 3.0 (De Novo Software, Los Angeles, CA) was used to calculate the ratio of the number S phase nuclei to the number of G1 phase nuclei with high nRNA content. A detailed description of the analyses is given in Porter and Bailey 2013.

Supplemental Information - Sampling Scales and Rates - Descriptive Information about the sampling spatial and temporal scales and rates.

Samples were taken from the southeastern Bering Sea and western Gulf of Alaska, and were conducted opportunistically over multiple years.

Supplemental Information - Error Analysis - Descriptive Information about the error analysis.

Supplemental Information - Provenance and Historical References -

Descriptive Information about the provenance, historical data, key information packaged elsewhere.

PORTER, S.M., and K.M. BAILEY. 2011. Assessing the condition of walleye pollock Theragra chalcogramma (Pallas) larvae using muscle-based flow cytometric cell cycle analysis. J. Exp. Mar. Biol. Ecol. 399:101-109.

PORTER, S. M., and K. M. BAILEY. 2013. Using measurements of muscle cell nuclear

RNA with flow cytometry to improve assessment of larval condition of walleye

pollock (Gadus chalcogrammus). Fish. Bull., U.S. 111:337-351.

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PORTER, S. M., and K. M. BAILEY. 2013. Using measurements of muscle cell nuclear

RNA with flow cytometry to improve assessment of larval condition of walleye

pollock (Gadus chalcogrammus). Fish. Bull., U.S. 111:337-351.

Data not generated by automated processing.

No archive currently supports this kind of data.

local and offsite backupd

A BD Biosciences Influx flow cytometer (BD Biosciences, San Jose, CA) was used to analyze each larva. The proportion of nuclei in the S, and G2/M phases was calculated for each larva with MultiCycle AV software, vers. 4.0 (Phoenix Flow Systems, San Diego, CA). FCS Express flow cytometry analysis software, vers. 3.0 (De Novo Software, Los Angeles, CA) was used to calculate the ratio of the number S phase nuclei to the number of G1 phase nuclei with high nRNA content. A detailed description of the analyses is given in Porter and Bailey 2013.