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NWFSC Metadata Library > EFS (Environmental and Fisheries Sciences) Divi... > Migrated from Resource Enhancement and Utilizat... > Light Experiment data - Snake River sockeye sal... >

Snake River Sockeye Salmon Light Dark Study

Entity (ENT)  |  Northwest Fisheries Science Center (NWFSC)
GUID: gov.noaa.nmfs.inport:51441  |  Updated: August 9, 2022  |  Published / External 
Summary
Item Identification
Entity Info
Data Attributes
Catalog Details

Summary

Abstract

This experiment compared the effect of indoor and outdoor lighting on the reproductive characteristics of sockeye salmon reared in a captive broodstock program. Indoor lighting was a mixture of natural lighting filtering through windows and artificial overhead lighting. Outdoor lighting was the product of sunlight passing through a translucent cover on the roof of a tensioned fabric greenhouse structure. Outdoor lighting in the greenhouse was about 80% of ambient sunlight and was about three orders of magnitude higher than the mixed lighting occurring inside the pole building. Fish in both experimental treatments were reared in identical indoor conditions from incubation to the time they would have naturally have reentered freshwater on their return migration from the sea. Maturing fish identified in maturity sorts conducted in late June-early July of each study year were systematically sorted into the two experimental treatments and stocked into the experimental raceways at Burley Creek Hatchery. In 2007, 2008, 2009, 2010 and 2011 these maturing fish were divided into eight similar groups with four groups being stocked into inside raceways and four groups being stocked into outside raceways. Data is also supplied for 2006 when only two inside and six outside raceways were available for the study. Except for lighting the fish were reared and handled similarly. When they were spawned in October and November all adults were assessed for fork length, weight, and ELISA values. The Julian date females spawned as well as the average weight of their green eggs (developed from an individual 10 egg weight sample), total green egg weight, and overall fecundity were determined and recorded. Males were assessed weekly for sperm production with the Julian Date of their first motile milt production recorded, along with their spermatocrit, and slaughtered milt weight. As each female's eggs were split in two lots and fertilized by two different males survival to eye (viability) is based on average survival of the two lots. This average viability was determined for both sexes.

Description

This experiment compared the effect of indoor and outdoor lighting on the reproductive characteristics of sockeye salmon reared in a captive broodstock program. Indoor lighting was a mixture of natural lighting filtering through windows and artificial overhead lighting. Outdoor lighting was the product of sunlight passing through a translucent cover on the roof of a tensioned fabric greenhouse structure. Outdoor lighting in the greenhouse was about 80% of ambient sunlight and was about three orders of magnitude higher than the mixed lighting occurring inside the pole building. Fish in both experimental treatments were reared in identical indoor conditions from incubation to the time they would have naturally have reentered freshwater on their return migration from the sea. Maturing fish identified in maturity sorts conducted in late June-early July of each study year were systematically sorted into the two experimental treatments and stocked into the experimental raceways at Burley Creek Hatchery. In 2007, 2008, 2009, 2010 and 2011 these maturing fish were divided into eight similar groups with four groups being stocked into inside raceways and four groups being stocked into outside raceways. Data is also supplied for 2006 when only two inside and six outside raceways were available for the study. Except for lighting the fish were reared and handled similarly. When they were spawned in October and November all adults were assessed for fork length, weight, and ELISA values. The Julian date females spawned as well as the average weight of their green eggs (developed from an individual 10 egg weight sample), total green egg weight, and overall fecundity were determined and recorded. Males were assessed weekly for sperm production with the Julian Date of their first motile milt production recorded, along with their spermatocrit, and slaughtered milt weight. As each female's eggs were split in two lots and fertilized by two different males survival to eye (viability) is based on average survival of the two lots. This average viability was determined for both sexes.

Entity Information

Entity Type
Spreadsheet

Data Attribute / Type Description
Sex
TEXT 		Sex of individual spawner with m equal male and f equal female.
Year
NUMBER 		The year in which the individual matured and was spawned.
Treatment
TEXT 		The experimental treatment applied to the fish. Inside designates fish undergoing maturation inside a building with low levels of natural and artificial lighting and Outside designates fish undergoing final maturation in a greenhouse with high levels of natural lighting filtered through a translucent overhead cover.
Raceway
TEXT 		Designates number or letter of raceway fish were held in. Inside treatment raceways are given a letter designation and outside treatment raceways are given a numerical designation. Raceway serves as unit of replication in this study.
mean Fork Length
NUMBER 		This is the average Fork Length of based on all fish of a given sex in the raceway. Fork Length is measured from tip of the snout to the fork of the tail in millimeters.
mean weight
NUMBER 		This is the average weight based on all fish of a given sex in a raceway. Weight is in grams.
mean ELISA
NUMBER 		This is the average kidney tissue enzyme-linked immunosorbent assay (ELISA) value for fish within a raceway. The value is reported as optical density (OD) based on the amount of light transmitted through a spectrophotometer.
Mean final spermatocrit
NUMBER 		This estimates the density of sperm in seminal fluid. It is a percentage value and represents the average taken for all males in a raceway on the last day they are sampled.
Mean final Sperm wt
NUMBER 		This is the weight in grams of all milt extracted from males on their terminal day.
Mean julian date first milt
NUMBER 		This is the julian date that a male first expressed milt. This is an average value for all males in a raceway.
Mean Julian date female spawned
NUMBER 		This is the julian date on which a female was considered to have mature eggs that were ready to be spawned. This is an average value based on the Julian date each female spawned.
mean % eyed egg survival
NUMBER 		This is the percentage of eggs that survived to the eyed stage. This is an average value for all fish of a given sex in the raceway.
Mean green egg wt
NUMBER 		This is the prefertilization weight, measure in grams, This number is determined for each female based on a 10 egg average. These numbers are then used to determine the average egg weight of all females in a raceway.
Mean eyed egg wt
NUMBER 		This is based on a sample of each females eyed eggs surviving to the eyed stage and is measured in grams. This value is then used in computing the average egg weight of all females in that raceway.
Mean fecundity
NUMBER 		This value is the mean number of eggs/female in the raceway.

Child Items

No Child Items for this record.

Contact Information

No contact information is available for this record.

Please contact the owner organization (NWFSC) for inquiries on this record.

Item Identification

Title: Snake River Sockeye Salmon Light Dark Study
Short Name: Snake River Sockeye Salmon Light Dark Study
Status: Planned
Abstract:

This experiment compared the effect of indoor and outdoor lighting on the reproductive characteristics of sockeye salmon reared in a captive broodstock program. Indoor lighting was a mixture of natural lighting filtering through windows and artificial overhead lighting. Outdoor lighting was the product of sunlight passing through a translucent cover on the roof of a tensioned fabric greenhouse structure. Outdoor lighting in the greenhouse was about 80% of ambient sunlight and was about three orders of magnitude higher than the mixed lighting occurring inside the pole building. Fish in both experimental treatments were reared in identical indoor conditions from incubation to the time they would have naturally have reentered freshwater on their return migration from the sea. Maturing fish identified in maturity sorts conducted in late June-early July of each study year were systematically sorted into the two experimental treatments and stocked into the experimental raceways at Burley Creek Hatchery. In 2007, 2008, 2009, 2010 and 2011 these maturing fish were divided into eight similar groups with four groups being stocked into inside raceways and four groups being stocked into outside raceways. Data is also supplied for 2006 when only two inside and six outside raceways were available for the study. Except for lighting the fish were reared and handled similarly. When they were spawned in October and November all adults were assessed for fork length, weight, and ELISA values. The Julian date females spawned as well as the average weight of their green eggs (developed from an individual 10 egg weight sample), total green egg weight, and overall fecundity were determined and recorded. Males were assessed weekly for sperm production with the Julian Date of their first motile milt production recorded, along with their spermatocrit, and slaughtered milt weight. As each female's eggs were split in two lots and fertilized by two different males survival to eye (viability) is based on average survival of the two lots. This average viability was determined for both sexes.

Entity Information

Entity Type: Spreadsheet
Active Version?: Yes
Schema Name: PARR
Description:

This experiment compared the effect of indoor and outdoor lighting on the reproductive characteristics of sockeye salmon reared in a captive broodstock program. Indoor lighting was a mixture of natural lighting filtering through windows and artificial overhead lighting. Outdoor lighting was the product of sunlight passing through a translucent cover on the roof of a tensioned fabric greenhouse structure. Outdoor lighting in the greenhouse was about 80% of ambient sunlight and was about three orders of magnitude higher than the mixed lighting occurring inside the pole building. Fish in both experimental treatments were reared in identical indoor conditions from incubation to the time they would have naturally have reentered freshwater on their return migration from the sea. Maturing fish identified in maturity sorts conducted in late June-early July of each study year were systematically sorted into the two experimental treatments and stocked into the experimental raceways at Burley Creek Hatchery. In 2007, 2008, 2009, 2010 and 2011 these maturing fish were divided into eight similar groups with four groups being stocked into inside raceways and four groups being stocked into outside raceways. Data is also supplied for 2006 when only two inside and six outside raceways were available for the study. Except for lighting the fish were reared and handled similarly. When they were spawned in October and November all adults were assessed for fork length, weight, and ELISA values. The Julian date females spawned as well as the average weight of their green eggs (developed from an individual 10 egg weight sample), total green egg weight, and overall fecundity were determined and recorded. Males were assessed weekly for sperm production with the Julian Date of their first motile milt production recorded, along with their spermatocrit, and slaughtered milt weight. As each female's eggs were split in two lots and fertilized by two different males survival to eye (viability) is based on average survival of the two lots. This average viability was determined for both sexes.

Data Attributes

Attribute Summary

Rubric Score Primary Key? Name Type Description
100
 Sex TEXT Sex of individual spawner with m equal male and f equal female.
100
 Year NUMBER The year in which the individual matured and was spawned.
100
 Treatment TEXT The experimental treatment applied to the fish. Inside designates fish undergoing maturation inside a building with low levels of natural and artificial lighting and Outside designates fish undergoing final maturation in a greenhouse with high levels of natural lighting filtered through a translucent overhead cover.
100
 Raceway TEXT Designates number or letter of raceway fish were held in. Inside treatment raceways are given a letter designation and outside treatment raceways are given a numerical designation. Raceway serves as unit of replication in this study.
100
 mean Fork Length NUMBER This is the average Fork Length of based on all fish of a given sex in the raceway. Fork Length is measured from tip of the snout to the fork of the tail in millimeters.
100
 mean weight NUMBER This is the average weight based on all fish of a given sex in a raceway. Weight is in grams.
100
 mean ELISA NUMBER This is the average kidney tissue enzyme-linked immunosorbent assay (ELISA) value for fish within a raceway. The value is reported as optical density (OD) based on the amount of light transmitted through a spectrophotometer.
100
 Mean final spermatocrit NUMBER This estimates the density of sperm in seminal fluid. It is a percentage value and represents the average taken for all males in a raceway on the last day they are sampled.
100
 Mean final Sperm wt NUMBER This is the weight in grams of all milt extracted from males on their terminal day.
100
 Mean julian date first milt NUMBER This is the julian date that a male first expressed milt. This is an average value for all males in a raceway.
100
 Mean Julian date female spawned NUMBER This is the julian date on which a female was considered to have mature eggs that were ready to be spawned. This is an average value based on the Julian date each female spawned.
100
 mean % eyed egg survival NUMBER This is the percentage of eggs that survived to the eyed stage. This is an average value for all fish of a given sex in the raceway.
100
 Mean green egg wt NUMBER This is the prefertilization weight, measure in grams, This number is determined for each female based on a 10 egg average. These numbers are then used to determine the average egg weight of all females in a raceway.
100
 Mean eyed egg wt NUMBER This is based on a sample of each females eyed eggs surviving to the eyed stage and is measured in grams. This value is then used in computing the average egg weight of all females in that raceway.
100
 Mean fecundity NUMBER This value is the mean number of eggs/female in the raceway.

Attribute Details

Sex

CC ID: 731308
Seq. Order: 1
Data Storage Type: TEXT
Required: No
Primary Key: No
Status: Active
Description:

Sex of individual spawner with m equal male and f equal female.
General Data Type: TEXT

Year

CC ID: 731309
Seq. Order: 2
Data Storage Type: NUMBER
Required: No
Primary Key: No
Status: Active
Description:

The year in which the individual matured and was spawned.
General Data Type: NUMBER

Treatment

CC ID: 731310
Seq. Order: 3
Data Storage Type: TEXT
Required: No
Primary Key: No
Status: Active
Description:

The experimental treatment applied to the fish. Inside designates fish undergoing maturation inside a building with low levels of natural and artificial lighting and Outside designates fish undergoing final maturation in a greenhouse with high levels of natural lighting filtered through a translucent overhead cover.
General Data Type: TEXT

Raceway

CC ID: 731311
Seq. Order: 4
Data Storage Type: TEXT
Required: No
Primary Key: No
Status: Active
Description:

Designates number or letter of raceway fish were held in. Inside treatment raceways are given a letter designation and outside treatment raceways are given a numerical designation. Raceway serves as unit of replication in this study.
General Data Type: TEXT

mean Fork Length

CC ID: 731312
Seq. Order: 5
Data Storage Type: NUMBER
Required: No
Primary Key: No
Status: Active
Description:

This is the average Fork Length of based on all fish of a given sex in the raceway. Fork Length is measured from tip of the snout to the fork of the tail in millimeters.
General Data Type: NUMBER

mean weight

CC ID: 731313
Seq. Order: 6
Data Storage Type: NUMBER
Required: No
Primary Key: No
Status: Active
Description:

This is the average weight based on all fish of a given sex in a raceway. Weight is in grams.
General Data Type: NUMBER

mean ELISA

CC ID: 731314
Seq. Order: 7
Data Storage Type: NUMBER
Required: No
Primary Key: No
Status: Active
Description:

This is the average kidney tissue enzyme-linked immunosorbent assay (ELISA) value for fish within a raceway. The value is reported as optical density (OD) based on the amount of light transmitted through a spectrophotometer.
General Data Type: NUMBER

Mean final spermatocrit

CC ID: 731315
Seq. Order: 8
Data Storage Type: NUMBER
Required: No
Primary Key: No
Status: Active
Description:

This estimates the density of sperm in seminal fluid. It is a percentage value and represents the average taken for all males in a raceway on the last day they are sampled.
General Data Type: NUMBER

Mean final Sperm wt

CC ID: 731316
Seq. Order: 9
Data Storage Type: NUMBER
Required: No
Primary Key: No
Status: Active
Description:

This is the weight in grams of all milt extracted from males on their terminal day.
General Data Type: NUMBER

Mean julian date first milt

CC ID: 731317
Seq. Order: 10
Data Storage Type: NUMBER
Required: No
Primary Key: No
Status: Active
Description:

This is the julian date that a male first expressed milt. This is an average value for all males in a raceway.
General Data Type: NUMBER

Mean Julian date female spawned

CC ID: 731318
Seq. Order: 11
Data Storage Type: NUMBER
Required: No
Primary Key: No
Status: Active
Description:

This is the julian date on which a female was considered to have mature eggs that were ready to be spawned. This is an average value based on the Julian date each female spawned.
General Data Type: NUMBER

mean % eyed egg survival

CC ID: 731319
Seq. Order: 12
Data Storage Type: NUMBER
Required: No
Primary Key: No
Status: Active
Description:

This is the percentage of eggs that survived to the eyed stage. This is an average value for all fish of a given sex in the raceway.
General Data Type: NUMBER

Mean green egg wt

CC ID: 731320
Seq. Order: 13
Data Storage Type: NUMBER
Required: No
Primary Key: No
Status: Active
Description:

This is the prefertilization weight, measure in grams, This number is determined for each female based on a 10 egg average. These numbers are then used to determine the average egg weight of all females in a raceway.
General Data Type: NUMBER

Mean eyed egg wt

CC ID: 731321
Seq. Order: 14
Data Storage Type: NUMBER
Required: No
Primary Key: No
Status: Active
Description:

This is based on a sample of each females eyed eggs surviving to the eyed stage and is measured in grams. This value is then used in computing the average egg weight of all females in that raceway.
General Data Type: NUMBER

Mean fecundity

CC ID: 731322
Seq. Order: 15
Data Storage Type: NUMBER
Required: No
Primary Key: No
Status: Active
Description:

This value is the mean number of eggs/female in the raceway.
General Data Type: NUMBER

Catalog Details

Catalog Item ID: 51441
GUID: gov.noaa.nmfs.inport:51441
Metadata Record Created By: Jeffrey W Cowen
Metadata Record Created: 2018-02-01 11:02+0000
Metadata Record Last Modified By: SysAdmin InPortAdmin
Metadata Record Last Modified: 2022-08-09 17:11+0000
Metadata Record Published: 2018-02-27
Owner Org: NWFSC
Metadata Publication Status: Published Externally
Do Not Publish?: N
Metadata Last Review Date: 2018-02-27
Metadata Review Frequency: 1 Year
Metadata Next Review Date: 2019-02-27